WebFluorescent Protein Labeling Additional notes on reducing high background staining Carefully prepare tissue sample. Damage to the tissue can cause diffuse staining. Prepare thinner sections if penetration of the detection reagents is insufficient. Optimize fixation. Each tissue antigen will react differently with different fixatives. WebAmong the five somatostatin receptors (SST1–SST5), SST4 is the least characterized, which is in part due to the lack of specific monoclonal antibodies. We generated a knockin mouse model that expresses a carboxyl-terminal SST4-eGFP fusion protein. In addition, we extensively characterized the novel rabbit monoclonal anti …
Fluorescent Immunohistology - 9 Tips For Successful Staining - Bitesize Bio
WebJan 1, 2024 · Fluorescent proteins, which require an external light source to stimulate emission of a different wavelength of light, are bright reporters and suitable to locate in which cells a gene is ... WebFluorescent In Situ Hybridization (Cell) Protocol Download; Fluorescent In Situ Hybridization (Formalin/PFA-fixed paraffin-embedded sections) enlarge this image Human lung, adenosquamous cell carcinoma (FFPE) stained with SYT Split FISH Probe. Human lung, adenosquamous cell carcinoma showed no SYT gene split. Protocol Download switch local account
Overview of Immunofluorescence (IF) Techniques - Cell …
WebIRIS11 Prestained Protein Ladder (11 pre-stained bands, 3-260 kDa) 500 μl: PMB01-0500: ... (12 bands, 100-3,000bps, fluorescent dye) 600 μl: DMF13-0100: OmniMARK 1K DNA Ladder RTU (13 bands, 100-10,000bps, fluorescent dye) ... (Paraffin-embedded tissue) 100 rxns: PDM01-0100: Mbead Buffy Coat Genomic DNA Kit: 100 rxns: PDM02-0100: WebTransgenes that use GFP-based proteins as a reporter provide a convenient way to follow protein expression over the course of development and disease progression in the … WebAdd a fluorescent label conjugated secondary antibody diluted with 1% serum in PBS (with or without 0.05-0.1% Triton X 100) and incubate at room temperature for 1-2 hours. Use the recommended dilution of the antibody specified on the datasheet. Note: For help selecting the optimal secondary antibody, please read our Secondary Antibody Handbook. switch load vs line